Ultrastructure of Azotobacter vinelandii.
نویسندگان
چکیده
Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not reported previously: intine fibrils, intine vesicles, intine membrane, and microtubules. The intine fibrils form a network in the gel-like homogeneous matrix of the CC2 layer. Intine vesicles which seem to originate in the cell wall complex of the central body are seen in the intine and exine of cysts. Analogous structures are found on vegetative cells. The intine is divided into two chemically distinct areas by the two-layered intine membrane. Microtubules, previously reported only in vegetative cells, were found in cysts.
منابع مشابه
Correlation of ultrastructure in Azotobacter vinelandii with nitrogen source for growth.
Azotobacter synthesizes an extensive internal membranous nework when grown with air (N(2)), i.e., under conditions when these bacteria fix nitrogen. Very slight quantities of internal membrane, concentrated mainly about the cell periphery, are formed when Azotobacter grows with fixed nitrogen, i.e., ammonia and amino acids. Compared to cells growing with ammonia, cells utilizing atmospheric nit...
متن کاملRestoration by ubiquinone of Azotobacter vinelandii reduced nicotinamide adenine dinucleotide oxidase activity.
Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone.
متن کاملTechnique for isolating phage for Azotobacter vinelandii.
An enrichment technique was developed whereby azotophage could readily be isolated after inoculation of soil sites with Azotobacter vinelandii.
متن کاملComplete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6
We report the complete genome sequences of Azotobacter vinelandii mutant strain CA6 and its parent wild-type strain, CA. When fixing nitrogen, strain CA6 displays slow growth and impaired molybdate uptake, tolerance to tungstates, and production of hydrogen gas, compared to results for strain CA. Comparing these genome sequences may provide a genetic basis for these mutant phenotypes.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 104 2 شماره
صفحات -
تاریخ انتشار 1970